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Extracellular vesicles (EVs) are important in inter-cellular communication as carriers of biological signals. Swarna's biological son Shubham dies of a drug overdose and Naira is blamed for hiding his drug addiction. Naira fulfills her parents dream of opening a dance academy and names it as "Akshara Dance Academy" as a tribute to her mother. Kartik reconciles with Manish and accepts Swarna as his mother after his mother's depression and hallucinations were revealed to be the reason for her suicide. Initially marrying for Naira's sake, he begins to love Keerti. Kartik hates his father Manish and his step-mother Swarna as he remarried her post his mother's death as he thinks that they were having an extra-marital affair in their office and were responsible for her suicide. Akshara dies by falling off a cliff in a car crash. Kartik confesses his love for Naira while latter is initially confused about her feelings but later realises her love for Kartik. 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RAR/RXR and PPAR/RXR Signaling in Spinal Cord Injury We propose that E1A functions as a cofactor by interacting with both TBP and RAR, thereby stabilizing the preinitiation complex. Furthermore, the region surrounding amino acid 178, partially overlapping with the TBP binding region, is involved in both binding to and activation by AF-2. The putative zinc finger region is crucial, probably as a consequence of interaction with TBP. Deletion analysis within this region indicated that the complete CRIII is needed for activation. This interaction is dependent on conserved region III (CRIII), the 13S mRNA-specific region of E1A. The activity of the hormone-dependent activation function 2 (AF-2) of RAR beta is upregulated by E1A, and an interaction between this region and E1A was observed, but not with AF-1 or AF-2 of RXR alpha. We now show that direct interaction between RAR and E1A is a requirement for retinoic acid-induced RAR beta 2 activation. The mechanism underlying this E1A requirement is largely unknown. For ligand-induced activation by the retinoic acid receptor-retinoid X receptor ( RAR-RXR) heterodimer, the RAR beta 2 promoter is dependent on the presence of E1A or E1A-like activity, since this promoter is activated by retinoic acid only in cells expressing such proteins. Transcription regulation by DNA-bound activators is thought to be mediated by a direct interaction between these proteins and TATA-binding protein (TBP), TFIIB, or TBP-associated factors, although occasionally cofactors or adapters are required. Adenovirus E1A functions as a cofactor for retinoic acid receptor beta ( RAR beta) through direct interaction with RAR beta. |
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